Journal: Journal of Immunology Research

Article Title: Production of Dermatophagoides farinae Having Low Bacterial Content Using Ampicillin

doi: 10.1155/2023/9024595

Figure Lengend Snippet: Induction of airway hyperreactivity with ampicillin treated- Dermatophagoides farinae (D.f. + AMP) and untreated D. farinae (D.f.) in vivo . (a) Airway resistance values. (b) Inflammatory cell infiltration in the lung. (c) Hematoxylin and eosin (H&E), periodic acid-schiff (PAS), and Masson's trichrome staining of the lung sections. (d) Expression of D. farinae -specific immunoglobulin (Ig)-E, IgG1, and IgG2a in mice. Data are presented as the mean ± standard deviation of at least three independent experiments. PBS, phosphate-buffered saline. ∗∗ p < 0.01, ∗ p < 0.05.

Article Snippet: Secondary antibodies were detected using biotinylated anti-mouse IgE (1 : 1,000; 408804; BioLegend, San Diego, CA, USA), biotinylated goat anti-mouse IgG1 (1 : 10,000; NBP1-69914B; Novus Biologicals, Littleton, CO, USA), and biotinylated goat anti-mouse IgG2a (1 : 10,000; NBP1-69915B; Novus Biologicals).

Techniques: In Vivo, Staining, Expressing, Standard Deviation, Saline

Journal: BioMed Research International

Article Title: Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System

doi: 10.1155/2021/2522305

Figure Lengend Snippet: Anisakis pegreffii extract (AE) treatment increases the expression of A. pegreffii -specific IgE, IgG1, and IgG2a. Measurement of A. pegreffii -specific IgE (1 : 1000), IgG1 (1 : 10000), and IgG2a (1 : 10000) levels by ELISA ( ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; n = 5 mice/group).

Article Snippet: The wells were then washed with wash buffer (0.05% Tween-20 in PBS) and incubated with the appropriate antibodies (50 μ L) for 2 h. The antibodies included biotinylated anti-mouse IgE (1 : 1000; 408804; BioLegend, San Diego, CA, USA), biotinylated goat anti-mouse IgG1 secondary antibody (1 : 10000; NBP1-69914B; Novus Biologicals, Littleton, CO, USA), and biotin goat anti-mouse IgG2a secondary antibody (1 : 10000; NBP1-69915B; Novus Biologicals).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay